Preparative LC System

Preparative HPLC systems 

Modular and robust prep HPLC platforms configured to your requirements.

Preparative HPLC systems in a modular format to accommodate purification ranging from a microgram to gram scale utilizing UV, ELSD, DAD, and/or MS as a fraction trigger and valve configurations to accommodate virtually any number of single or multiple column schemes.

The systems's wide flow rate range (from the 333 and 334 Pumps) allows for both semi-preparative and preparative separations. High pumping power accommodates a wide range of preparative column sizes. All injection modules feature a continuous flow path design. The system can accommodate large injection volumes with minimal sample waste and carryover. The new rinse station system incorporates a flowing jet wash and allows the use of up to two different rinse solvents.

  • Low dwell volume allows fast, reproducible gradients
  • Off bed collection racks allow for true large-scale fraction collection into vessels of your choice without sacrificing bed space
  • Incorporate Gilson's System Organizers for stacking to conserve bench space
  • Injection of up to 15 g per run depending on column dimensions
  • The 30X Pump Series configured as injection pumps can inject volumes up to several liters to maximize sample loading on preparative HPLC columns
  • Separate injection and collection pathways that eliminate contamination of fractions from the injection process
  • The solvent valve allows for immediate access of up to five off bed solvent reservoirs
  • The GX Direct Injection Module features an innovative injection port design that attaches directly to the injection valve reducing void volumes and minimizing carryover associated with calibrated connection tubing
  • The GX Direct Injection Module offers various sample loop sizes providing the largest sample volume range on the market today

333/334 Prep-Scale HPLC Pumps

Your delivery solution for preparative chromatography. Capable of fast separations with high-efficiency columns in normal and reverse-phase modes. Choose the 333 Master Pump for isocratic applications or a 333/334 combination for binary gradient applications.

  • Milligram-to-gram level sample mass throughput per injection; up to 15 g depending on column size and loading capacity
  • Composition gradient with high-pressure mixing: 333-334 binary system (third pump for ternary). Solvent selection on each pump with addition of four-solvent valve (up to eight solvents)
  • Ability to operate additional pumps in parallel to double the flow rate up to 400 mL/min
  • Control from 333 master pump: flow-rate gradient, four-solvent selection and step gradient (additional valve), composition gradient (one or two additional pumps), sample-injection pump, UV/VIS detector and electrical input/output contacts with any other associated equipment
  • Quiet operation, durable design
  • Control 33X Pumps and an entire preparative HPLC system via TRILUTION® LC Software
  • Stackable to conserve bench space ( More information)


TRILUTION LC is an easy-to-use, walk-up software in which an application can be up and running in fewer than five steps. It features conditional logic fraction collection, robust worry-free slope collection, 100% accurate fraction simulation that gives you a visual of how compounds will be collected, off-scale peak collection, effortless graphical drag and drop user interface, and graphical sample tracing.

Saving time and resources allows you to keep up with your laboratories' growing demands

TRILUTION LC conditional logic collection capabilities allow chemists to optimize purification, reducing the number of fractions that are collected, needed to be re-analyzed and handled. TRILUTION LC allows you to:

  • Collect specific peaks using conditional; AND, AND/OR, and OR statements
  • Apply conditional statements to the same detector signal using a combination of slope and level collection to obtain the highest possible purity
  • Collect based on data from several different detectors: UV, DAD, ELSD, and MS
  • Apply the ultimate efficiency tool and collect fractions based on UV and target masses 
( More information)