Fraction Collectors

FC203/ FC204 Fraction Collectors

Advanced fraction collection solutions with a complete line of stand-alone and software controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between.

The FC 203B and FC 204 Fraction Collectors are ideal instruments to integrate with any HPLC system. Capable of collecting into a variety of vessels these fraction collectors can also be used in a stand-alone
operation using time, drop or peak based collection.

Features & Benefits
  • Choose the compact FC 203B for lower capacity or the FC 204 for large volumes and capacities
  • Accepts a wide variety of collection vessels, including microplates, vials, and tubes
  • In peak mode, the collector monitors a detector signal and identifies peaks using either an adaptive slope algorithm or a specified millivolt threshold
  • Up to ten programmable time windows can be added for collecting specified samples while discarding the column void volume, peaks of no interest and equilibration volumes
  • Operate as a stand-alone instrument with incorporated keypad or control through TRILUTION® LC Software
  • 3-way diverter valve prevents contamination of collected fractions (standard on the FC 204, optional on the FC 203B)
  • Contact closure inputs are available to remotely start, advance or stop fraction collection—compatible with most HPLC systems
  • Multi-column adapters for collecting up to 18 separate channels (9 on the FC 203B)
  • Compact size; fits in most fume hoods

Manufacturing Standards Meets applicable safety and EMC certification standards; UL and CE certified
3-way Valves FC 203B/204: 3-port, PTFE, 67 µL internal volume, 3.5 µL dead volume from common port to the normally closed port, up to 20 mL/min with standard or large bore needle
FC 204: 3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 20 mL/min with standard or large bore needle
Communication Interface GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices
Data Acquisition An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC
Detector Input ±100 mV full scale
Drop Counting Up to 9,999 drops per fraction; maximum rate: 20 drops /sec
Environmental Conditions Indoor use
Altitude: up to 2000 m
Temperature range: 5–40°C
Air pressure: 75–105 kPa
Pollution degree: 1 or 2, in accordance with IEC 66
Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C
Event Marker 100 ms pulse
Front Panel Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0–9 numeric hard keys; four soft keys
Maximum Collection Volume/Tube FC 203B: 20 mL (28 x 60 mm scintillation vials with optional Code 24 rack)
FC 204: 32 mL (18 x 180 mm tubes or 25 mL (18 x 150 mm)
Maximum Fractions FC 203B: 128 (12 x 75 mm tubes with optional Code 14 rack)
FC 204: Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)
Multiple-Column Collection Simultaneous collection from up to 8 (FC 203B) or up to 18 (FC 204) columns with installation of optional multiple column adaptor(s)
Multiple-Cycle Operation Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes
Number of Racks FC 203B: Holds one rack
FC 204: Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)
Operating Modes Drop, Manual, Peak + Drop, Peak + Time, Time
Peak Detection Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value
Peak parameters: peak height or level, in mV and peak width at half-height, in minutes
Minimum peak height for fractionation: 0.1 mV full scale
Detector-collector delay: 0.01 minute increments
Power Requirements Frequency: 50–60 Hz
Voltage: 100–120 or 220–240V; mains voltage fluctuations not to exceed ±10% of the nominal voltage
Current rating: 0.5A for 100–120V, or 0.25A for 220–240V
Programmable Time Units From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution
Software Via TRILUTION® LC Software
Time-Based Programming Up to 10 collection windows and 10 drain steps in any mode
Tube Change Time 100—250 ms (FC 203B) or 100—720 ms (FC 204), center to center, depending on rack type
Dimensions (w x d x h) FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in.)
FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)
Weight FC 203B: 5 kg (11 lbs.)
FC 204: 10.9 kg (24 lbs.)